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Product Info Summary
| SKU: | EK0925 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse DKK-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0925
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse DKK-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Dkk1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse DKK-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0925)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: S30-H272
Sensitivity
<15 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0925 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0925 is reactive to Dkk1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0925 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Dkk-1
Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0925-CAP | Anti-Mouse Dkk1 Pre-coated 96-well strip microplate | 1 |
| EK0925-ST | Mouse Dkk1 Standard | 2 vials, 10 ng/tube |
| EK0925-DA | Mouse Dkk1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.013 | 0.059 | 0.096 | 0.168 | 0.372 | 0.681 | 1.249 | 1.972 |
Data Example Images
Click image to see more details
Mouse DKK1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Dkk-1, Dilution ratio of 1:20, concentration in serum and plasma is 1156-33076 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse DKK-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 97 | 425 | 1418 | 94 | 417 | 1519 |
| Standard deviation | 6.4 | 30.17 | 68.06 | 7.4%9 | 30.85 | 88.1 |
| CV (%) | 6.6% | 7.1% | 4.8% | 6.8% | 7.4% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Dkk-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 97 | 97 | 101 | 103 | 99 | 2.59 | 2.6% |
| Sample 2 | 425 | 361 | 363 | 388 | 384 | 25.82 | 6.7% |
| Sample 3 | 1418 | 1320 | 1228 | 1302 | 1317 | 67.74 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For Dkk1 (Source: Uniprot.Org, NCBI)
Gene Name
Dkk1
Full Name
Dickkopf-related protein 1
Weight
29.298kDa
Superfamily
dickkopf family
Alternative Names
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like Dkk1|mdkk-1|dickkopf WNT signaling pathway inhibitor 1|dickkopf-related protein 1|dickkopf homolog 1|dickkopf-1|dickkopf-like protein 1|dkk-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Dkk1, check out the Dkk1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Dkk1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse DKK-1 ELISA Kit PicoKine® (EK0925)
Hello CJ!
EK0925 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
SIRT6 Deficiency Culminates in Low-Turnover Osteopenia
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Customer Q&As
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11 Customer Q&As for Mouse DKK-1 ELISA Kit PicoKine®
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-10-05
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-10-05
Question
Q: if the enzyme conjugated Dkk-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Dkk-1 antigen?
G. Miller
Verified customer
Asked: 2019-05-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-05-16
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-04-17
Question
Q: which procedure should I follow in order to thaw whole blood sample for Dkk-1 ELISA after freezing?
D. Sunder
Verified customer
Asked: 2018-08-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Dkk-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-08-13
Question
Does EK0925 cross-react with rat?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
EK0925 cross-reacts with rat. There is a 90% reactivity with recombinant rat protein.
Boster Scientific Support
Answered: 2018-07-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
J. Allen
Verified customer
Asked: 2018-01-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-01-20
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
M. Nelson
Verified customer
Asked: 2017-06-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-06-08
Question
Q: What is the optimal O.D. value for Dkk-1 ELISA kit? I performed your Dkk-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Dkk-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-09-17
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Dkk-1 ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Dkk-1 that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2016-09-17
Question
Q: how do I analyze ELISA data? I measured Dkk-1 level in serum.
V. Liu
Verified customer
Asked: 2016-05-21
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-05-21
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Dkk-1 using the EK0925 Mouse Dkk-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-01-05
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2016-01-05
Question
Q: can I use citrate plasma as samples in Mouse Dkk-1 Picokine® ELISA Kit (Catalog # EK0925)?
Verified Customer
Verified customer
Asked: 2015-08-31
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Dkk-1 causing disruption of its protein structure. Dkk-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Dkk-1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-08-31
