Product Info Summary
SKU: | EK0792 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Mouse |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse IL-17D ELISA Kit PicoKine®
SKU/Catalog Number
EK0792
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-17D ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il17d in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-17D ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0792)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A25-R205
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0792 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0792 is reactive to IL17D in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0792 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-17D
Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK0792-CAP | Anti-Mouse IL17D Pre-coated 96-well strip microplate | 1 |
EK0792-ST | Mouse IL17D Standard | 2 vials, 10 ng/tube |
EK0792-DA | Mouse IL17D Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.042 | 0.106 | 0.158 | 0.253 | 0.460 | 0.883 | 1.530 | 2.088 |
Data Example Images
Click image to see more details
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-17D, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 62.5 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-17D ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 147 | 775 | 1330 | 157 | 700 | 1222 |
Standard deviation | 5.88 | 51.15 | 98.42 | 8.79 | 50.4 | 97.76 |
CV (%) | 4% | 6.6% | 7.4% | 5.6% | 7.2% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-17D in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 147 | 150 | 156 | 137 | 147 | 6.87 | 4.6% |
Sample 2 | 775 | 677 | 680 | 767 | 724 | 46.34 | 6.4% |
Sample 3 | 1330 | 1370 | 1170 | 1232 | 1275 | 78.93 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For IL17D (Source: Uniprot.Org, NCBI)
Gene Name
IL17D
Full Name
Interleukin-17D
Weight
21.893kDa
Superfamily
IL-17 family
Alternative Names
FLJ30846; IL17D; IL-17D; IL-17Dinterleukin 27; IL-22; IL-27interleukin-17D; IL27interleukin-27; interleukin 17D; Interleukin-27 IL17D IL-17D interleukin 17D interleukin-17D
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL17D, check out the IL17D Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL17D: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-17D ELISA Kit PicoKine® (EK0792)
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No publications found for EK0792
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
6 Customer Q&As for Mouse IL-17D ELISA Kit PicoKine®
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-09-20
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-09-20
Question
Q: can I use citrate plasma as samples in Mouse IL-17D Picokine® ELISA Kit (Catalog # EK0792)?
Verified Customer
Verified customer
Asked: 2020-06-27
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-17D causing degradation of its protein structure. IL-17D may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-17D ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-06-27
Question
Q: if the enzyme conjugated IL-17D antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-17D antigen?
Verified Customer
Verified customer
Asked: 2020-01-18
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-01-18
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-04-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-04-05
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL-17D in Mouse cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
M. Kapoor
Verified customer
Asked: 2019-04-05
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-17D ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-04-05
Question
Q: how to thaw whole blood sample for IL-17D ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-07-08
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17D for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-07-08