Mouse CD73/NT5E ELISA Kit PicoKine®

Product Name

Mouse CD73/NT5E ELISA Kit PicoKine®

View all 5& ELISA kits

SKU/Catalog Number

EK2131

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD73/NT5E ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Nt5e in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CD73/NT5E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2131)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: W29-S551

Sensitivity

<20 pg/ml

Assay Range

0.156 ng/ml - 10 ng/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2131 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2131 is reactive to NT5E in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2131 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of 5&

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Kit Components

Catalog Number	Description	Quantity
EK2131-CAP	Anti-Mouse NT5E Pre-coated 96-well strip microplate	1
EK2131-ST	Mouse NT5E Standard	2 vials, 10 ng/tube
EK2131-DA	Mouse NT5E Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(ng/ml)	0	0.156	0.312	0.625	1.25	2.5	5	10
O.D.	0.019	0.138	0.241	0.334	0.409	0.719	1.304	1.902

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect 5&, Dilution ratio of 1:10, concentration in serum and plasma is around 30 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CD73/NT5E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of 5& in ELISA kits from four different production batches/lots.

Gene/Protein Information For NT5E (Source: Uniprot.Org, NCBI)

Alternative Names

5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase NT5E CALJA, CD73, E5NT, NT, NT5, NTE, eN, eNT 5-nucleotidase ecto 5-nucleotidase|5-NT|Purine 5-Prime-Nucleotidase|ecto-5-nucleotidase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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