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Product Info Summary
| SKU: | LS116535 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of MAS-related GPR, member F (MRGPRF), transcript variant 1 |
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Product info
Product Name
MRGPRF (NM_001098515) Human Over-expression Lysate
SKU/Catalog Number
LS116535
Size
100 µg
Description
Transient overexpression lysate of MAS-related GPR, member F (MRGPRF), transcript variant 1
Storage & Handling
Cite This Product
MRGPRF (NM_001098515) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS116535)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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MRGPRF Human Over-expression Lysates NM_001098515.
Western blot validation of overexpression lysate (LS116535) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MRGPRF (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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MRGPRF Human Over-expression Lysates NM_145015.
Western blot validation of overexpression lysate (LS116535) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MRGPRF (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for MRGPRF (Source: Uniprot.org, NCBI)
Gene Name
MRGPRF
Full Name
Mas-related G-protein coupled receptor member F
Weight
38.171kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
FLJ16111; FLJ29034; FLJ40998; FLJ53714; G protein-coupled receptor 140; G protein-coupled receptor 168; G protein-coupled receptor MrgF; GPR140; GPR168; G-protein coupled receptor 140; G-protein coupled receptor 168; mas-related G protein-coupled MRGF; Mas-related gene F protein; MAS-related GPR, member F; mas-related G-protein coupled receptor member F; MGC21621; mrgF; MRGFDKFZp586B2122; MRGPRF; RTA; seven transmembrane helix receptor MRGPRF GPR140, GPR168, MRGF, RTA MAS related GPR family member F mas-related G-protein coupled receptor member F|G protein-coupled receptor MrgF|G-protein coupled receptor 140|G-protein coupled receptor 168|MAS-related GPR, member F|Mas-related G protein-coupled receptor F|mas-related G protein-coupled MRGF|mas-related gene F protein|seven transmembrane helix receptor
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on MRGPRF, check out the MRGPRF Infographic
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In this infographic, you will see the following information for MRGPRF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For MRGPRF (NM_001098515) Human Over-expression Lysate (LS116535)
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