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Product Info Summary
| SKU: | LS079661 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of nei endonuclease VIII-like 1 (E. coli) (NEIL1) |
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Product info
Product Name
NEIL1 (NM_024608) Human Over-expression Lysate
SKU/Catalog Number
LS079661
Size
100 µg
Description
Transient overexpression lysate of nei endonuclease VIII-like 1 (E. coli) (NEIL1)
Storage & Handling
Cite This Product
NEIL1 (NM_024608) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS079661)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information for Neil1 (Source: Uniprot.org, NCBI)
Gene Name
Neil1
Full Name
Endonuclease 8-like 1
Weight
43.586kDa
Superfamily
FPG family
Alternative Names
DNA-(apurinic or apyrimidinic site) lyase Neil1; EC 3.2.2.-; EC 4.2.99.18; endonuclease 8-like 1; endonuclease VIII; Endonuclease VIII-like 1; FLJ22402; FPG1DNA glycosylase/AP lyase Neil1; hFPG1; NEH1; nei endonuclease VIII-like 1 (E. coli); Nei homolog 1; NEI1; Nei-like protein 1 Neil1|2810450N13Rik, NEH1, Nei1|nei endonuclease VIII-like 1 (E. coli)|endonuclease 8-like 1|DNA glycosylase/AP lyase Neil1|DNA-(apurinic or apyrimidinic site) lyase Neil1|endonuclease VIII|endonuclease VIII-like 1|nei homolog 1|nei-like protein 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Neil1, check out the Neil1 Infographic
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In this infographic, you will see the following information for Neil1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For NEIL1 (NM_024608) Human Over-expression Lysate (LS079661)
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