NHP2L1 (SNU13) (NM_005008) Human Over-expression Lysate

Lysate of SNU13 over-expression cell lines, WB control

Transient overexpression lysate of NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) (NHP2L1), transcript variant 1

Product Info Summary

SKU: LS004809
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) (NHP2L1), transcript variant 1

Product Name

NHP2L1 (SNU13) (NM_005008) Human Over-expression Lysate

See all SNU13 products

SKU/Catalog Number

LS004809

Size

100 µg

Description

Transient overexpression lysate of NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) (NHP2L1), transcript variant 1

Storage & Handling

Cite This Product

NHP2L1 (SNU13) (NM_005008) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS004809)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SNU13 (Source: Uniprot.org, NCBI)

Gene Name

SNU13

Full Name

NHP2-like protein 1

Weight

14.174kDa

Superfamily

eukaryotic ribosomal protein eL8 family

Alternative Names

NHP2-like protein 1 SNU13 15.5K, FA-1, FA1, NHP2L1, NHPX, OTK27, SNRNP15-5, SPAG12, SSFA1 small nuclear ribonucleoprotein 13 NHP2-like protein 1|NHP2 non-histone chromosome protein 2-like 1|SNU13 homolog, small nuclear ribonucleoprotein (U4/U6.U5)|U4/U6.U5 tri-snRNP 15.5 kDa protein|[U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein|high mobility group-like nuclear protein 2 homolog 1|non-histone chromosome protein 2-like 1|sperm specific antigen 1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on SNU13, check out the SNU13 Infographic

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In this infographic, you will see the following information for SNU13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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