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Product Info Summary
| SKU: | LS023057 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of nicotinamide nucleotide adenylyltransferase 2 (NMNAT2), transcript variant 2 |
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Product info
Product Name
NMNAT2 (NM_170706) Human Over-expression Lysate
SKU/Catalog Number
LS023057
Size
100 µg
Description
Transient overexpression lysate of nicotinamide nucleotide adenylyltransferase 2 (NMNAT2), transcript variant 2
Storage & Handling
Cite This Product
NMNAT2 (NM_170706) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023057)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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NMNAT2 Human Over-expression Lysates NM_015039.
Western blot validation of overexpression lysate (LS023057) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NMNAT2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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NMNAT2 Human Over-expression Lysates NM_170706.
Western blot validation of overexpression lysate (LS023057) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NMNAT2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for NMNAT2 (Source: Uniprot.org, NCBI)
Gene Name
NMNAT2
Full Name
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
Weight
34.439kDa
Superfamily
eukaryotic NMN adenylyltransferase family
Alternative Names
C1orf15; chromosome 1 open reading frame 15; EC 2.7.7.1; EC 2.7.7.18; KIAA0479MGC2756; NaMN adenylyltransferase 2; nicotinamide mononucleotide adenylyltransferase 2; nicotinamide nucleotide adenylyltransferase 2; Nicotinate-nucleotide adenylyltransferase 2; NMN adenylyltransferase 2; NMNAT2; NMNAT-2; PNAT2; pyridine nucleotide adenylyltransferase 2 NMNAT2 C1orf15, PNAT2 nicotinamide nucleotide adenylyltransferase 2 nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2|NMN adenylyltransferase 2|NMN/NaMN adenylyltransferase 2|NaMN adenylyltransferase 2|nicotinamide mononucleotide adenylyltransferase 2|nicotinate-nucleotide adenylyltransferase 2|pyridine nucleotide adenylyltransferase 2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on NMNAT2, check out the NMNAT2 Infographic
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In this infographic, you will see the following information for NMNAT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For NMNAT2 (NM_170706) Human Over-expression Lysate (LS023057)
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