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Product Info Summary
| SKU: | LS010101 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of nucleotide binding protein 2 (MinD homolog, E. coli) (NUBP2) |
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Product info
Product Name
NUBP2 (NM_012225) Human Over-expression Lysate
SKU/Catalog Number
LS010101
Size
100 µg
Description
Transient overexpression lysate of nucleotide binding protein 2 (MinD homolog, E. coli) (NUBP2)
Storage & Handling
Cite This Product
NUBP2 (NM_012225) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010101)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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NUBP2 Human Over-expression Lysates NM_012225.
Western blot validation of overexpression lysate (LS010101) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NUBP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for NUBP2 (Source: Uniprot.org, NCBI)
Gene Name
NUBP2
Full Name
Cytosolic Fe-S cluster assembly factor NUBP2
Weight
28.825kDa
Superfamily
Mrp/NBP35 ATP-binding proteins family
Alternative Names
CFD1C447E6.1 (nucleotide binding protein 1 (E.coli MinD like) ); cytosolic Fe-S cluster assembly factor NUBP2; homolog of yeast cytosolic Fe-S cluster deficient 1; NBP 2; NUBP1; nucleotide binding protein 2 (E.coli MinD like); nucleotide binding protein 2 (MinD homolog, E. coli); Nucleotide-binding protein 2 NUBP2 CFD1, CIAO6, NBP 2, NUBP1 nucleotide binding protein 2 cytosolic Fe-S cluster assembly factor NUBP2|homolog of yeast cytosolic Fe-S cluster deficient 1|nucleotide binding protein 2 (MinD homolog, E. coli)
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on NUBP2, check out the NUBP2 Infographic
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In this infographic, you will see the following information for NUBP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For NUBP2 (NM_012225) Human Over-expression Lysate (LS010101)
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No publications found for LS010101
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