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Product Info Summary
| SKU: | LS005028 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of purinergic receptor P2Y, G-protein coupled, 1 (P2RY1) |
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Product info
Product Name
P2Y1 (P2RY1) (NM_002563) Human Over-expression Lysate
SKU/Catalog Number
LS005028
Size
100 µg
Description
Transient overexpression lysate of purinergic receptor P2Y, G-protein coupled, 1 (P2RY1)
Storage & Handling
Cite This Product
P2Y1 (P2RY1) (NM_002563) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005028)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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P2Y1 (P2RY1) Human Over-expression Lysates NM_002563.
Western blot validation of overexpression lysate (LS005028) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with P2RY1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for P2RY1 (Source: Uniprot.org, NCBI)
Gene Name
P2RY1
Full Name
P2Y purinoceptor 1
Weight
42.072kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
ATP receptor; P2 purinoceptor subtype Y1; P2RY1; P2Y purinoceptor 1; P2Y1; P2Y1platelet ADP receptor; purinergic receptor P2Y, G-protein coupled, 1; Purinergic receptor P2RY1 P2Y1, SARCC purinergic receptor P2Y1 P2Y purinoceptor 1|ADP receptor|ATP receptor|P2 purinoceptor subtype Y1|platelet ADP receptor|purinergic receptor P2Y, G-protein coupled, 1|suppressing androgen receptor in renal cell carcinoma
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on P2RY1, check out the P2RY1 Infographic
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In this infographic, you will see the following information for P2RY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For P2Y1 (P2RY1) (NM_002563) Human Over-expression Lysate (LS005028)
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