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Product Info Summary
| SKU: | LS005223 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of phosphoglycerate mutase 1 (brain) (PGAM1) |
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Product info
Product Name
PGAM1 (NM_002629) Human Over-expression Lysate
SKU/Catalog Number
LS005223
Size
100 µg
Description
Transient overexpression lysate of phosphoglycerate mutase 1 (brain) (PGAM1)
Storage & Handling
Cite This Product
PGAM1 (NM_002629) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005223)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PGAM1 Human Over-expression Lysates NM_002629.
Western blot validation of overexpression lysate (LS005223) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PGAM1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PGAM1 (Source: Uniprot.org, NCBI)
Gene Name
PGAM1
Full Name
Phosphoglycerate mutase 1
Weight
28.804kDa
Superfamily
phosphoglycerate mutase family
Alternative Names
BPG-dependent PGAM 1; EC 3.1.3.13; EC 5.4.2.1; EC 5.4.2.4; PGAMA; PGAM-Bphosphoglycerate mutase 1; phosphoglycerate mutase 1 (brain); phosphoglycerate mutase A, nonmuscle form; Phosphoglycerate mutase isozyme B PGAM1 HEL-S-35, PGAM-B, PGAMA phosphoglycerate mutase 1 phosphoglycerate mutase 1|BPG-dependent PGAM 1|epididymis secretory protein Li 35|phosphoglycerate mutase 1 (brain)|phosphoglycerate mutase A, nonmuscle form|phosphoglycerate mutase isozyme B
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PGAM1, check out the PGAM1 Infographic
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In this infographic, you will see the following information for PGAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PGAM1 (NM_002629) Human Over-expression Lysate (LS005223)
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