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Product Info Summary
| SKU: | LS005287 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of phosphoinositide-3-kinase, class 2, beta polypeptide (PIK3C2B) |
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Product info
Product Name
PIK3C2B (NM_002646) Human Over-expression Lysate
See all PI 3-Kinase C2 beta products
SKU/Catalog Number
LS005287
Size
100 µg
Description
Transient overexpression lysate of phosphoinositide-3-kinase, class 2, beta polypeptide (PIK3C2B)
Storage & Handling
Cite This Product
PIK3C2B (NM_002646) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005287)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PIK3C2B Human Over-expression Lysates NM_002646.
Western blot validation of overexpression lysate (LS005287) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PIK3C2B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PIK3C2B (Source: Uniprot.org, NCBI)
Gene Name
PIK3C2B
Full Name
Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta
Weight
184.768kDa
Superfamily
PI3/PI4-kinase family
Alternative Names
C2-PI3K; C2-PI3KDKFZp686G16234; EC 2.7.1; EC 2.7.1.154; phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit beta; Phosphoinositide 3-kinase-C2-beta; phosphoinositide-3-kinase, class 2, beta polypeptide; PI 3Kinase C2 beta; PI 3-Kinase C2 beta; PI3K-C2beta; PI3K-C2-beta; PIK3C2B; PTDINS-3-kinase C2 beta; PtdIns-3-kinase C2 subunit beta PIK3C2B C2-PI3K phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta|PI3K-C2-beta|PI3K-C2beta|PTDINS-3-kinase C2 beta|phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide|phosphoinositide 3-kinase-C2-beta|phosphoinositide-3-kinase, class 2, beta polypeptide|ptdIns-3-kinase C2 subunit beta
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PIK3C2B, check out the PIK3C2B Infographic
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In this infographic, you will see the following information for PIK3C2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PIK3C2B (NM_002646) Human Over-expression Lysate (LS005287)
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