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Product Info Summary
| SKU: | LS005305 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of phosphatidylinositol-5-phosphate 4-kinase, type II, alpha (PIP4K2A) |
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Product info
Product Name
PIP5K2 alpha (PIP4K2A) (NM_005028) Human Over-expression Lysate
SKU/Catalog Number
LS005305
Size
100 µg
Description
Transient overexpression lysate of phosphatidylinositol-5-phosphate 4-kinase, type II, alpha (PIP4K2A)
Storage & Handling
Cite This Product
PIP5K2 alpha (PIP4K2A) (NM_005028) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005305)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PIP5K2 alpha (PIP4K2A) Human Over-expression Lysates NM_005028.
Western blot validation of overexpression lysate (LS005305) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PIP4K2A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PIP4K2A (Source: Uniprot.org, NCBI)
Gene Name
PIP4K2A
Full Name
Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
Weight
46.225kDa
Alternative Names
1-phosphatidylinositol-4-phosphate kinase; 1-phosphatidylinositol-4-phosphate-5-kinase; 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha; Diphosphoinositide kinase 2-alpha; EC 2.7.1; EC 2.7.1.149; FLJ13267; phosphatidylinositol-4-phosphate 5-kinase, type II, alpha; Phosphatidylinositol-5-phosphate 4-kinase type II alpha; phosphatidylinositol-5-phosphate 4-kinase type-2 alpha; phosphatidylinositol-5-phosphate 4-kinase, type II, alpha; PI(5)P 4-kinase type II alpha; PIP4KII-alpha; PIP5K2; PIP5K2API5P4KA; PIP5KIIA; PIP5KIIalpha; PIP5KII-alpha; PIP5KIII; PIPK; PtdIns(4)P-5-kinase B isoform; Ptd PIP4K2A PI5P4KA, PIP5K2A, PIP5KII-alpha, PIP5KIIA, PIPK phosphatidylinositol-5-phosphate 4-kinase type 2 alpha phosphatidylinositol 5-phosphate 4-kinase type-2 alpha|1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha|1-phosphatidylinositol-4-phosphate kinase|1-phosphatidylinositol-4-phosphate-5-kinase|PI(5)P 4-kinase type II alpha|PI5P4Kalpha|PIP4KII-alpha|PIP5KIII|PIP5KIIalpha|diphosphoinositide kinase 2-alpha|phosphatidylinositol 5-phosphate 4-kinase type II alpha|phosphatidylinositol-4-phosphate 5-kinase, type II, alpha
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PIP4K2A, check out the PIP4K2A Infographic
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In this infographic, you will see the following information for PIP4K2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PIP5K2 alpha (PIP4K2A) (NM_005028) Human Over-expression Lysate (LS005305)
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