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Product Info Summary
| SKU: | LS005324 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of pleiomorphic adenoma gene 1 (PLAG1), transcript variant 1 |
Product info
Product Name
PLAG1 (NM_002655) Human Over-expression Lysate
SKU/Catalog Number
LS005324
Size
100 µg
Description
Transient overexpression lysate of pleiomorphic adenoma gene 1 (PLAG1), transcript variant 1
Storage & Handling
Cite This Product
PLAG1 (NM_002655) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005324)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PLAG1 Human Over-expression Lysates NM_001114634.
Western blot validation of overexpression lysate (LS005324) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PLAG1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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PLAG1 Human Over-expression Lysates NM_001114635.
Western blot validation of overexpression lysate (LS005324) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PLAG1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
PLAG1 Human Over-expression Lysates NM_002655.
Western blot validation of overexpression lysate (LS005324) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PLAG1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PLAG1 (Source: Uniprot.org, NCBI)
Gene Name
PLAG1
Full Name
Zinc finger protein PLAG1
Weight
55.909kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
COL1A2/PLAG1 fusion; Pleiomorphic adenoma gene 1 protein; pleiomorphic adenoma gene 1; SGPA; zinc finger protein PLAG1; ZNF912 PLAG1 PSA, SGPA, SRS4, ZNF912 PLAG1 zinc finger zinc finger protein PLAG1|COL1A2/PLAG1 fusion|HAS2/PLAG1 fusion|pleiomorphic adenoma gene 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PLAG1, check out the PLAG1 Infographic
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In this infographic, you will see the following information for PLAG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PLAG1 (NM_002655) Human Over-expression Lysate (LS005324)
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