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Product Info Summary
| SKU: | LS063977 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of PR domain containing 15 (PRDM15), transcript variant 1 |
Product info
Product Name
PRDM15 (NM_022115) Human Over-expression Lysate
SKU/Catalog Number
LS063977
Size
100 µg
Description
Transient overexpression lysate of PR domain containing 15 (PRDM15), transcript variant 1
Storage & Handling
Cite This Product
PRDM15 (NM_022115) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS063977)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PRDM15 Human Over-expression Lysates NM_022115.
Western blot validation of overexpression lysate (LS063977) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PRDM15 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PRDM15 (Source: Uniprot.org, NCBI)
Gene Name
PRDM15
Full Name
PR domain zinc finger protein 15
Weight
169.269kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
C21orf83; chromosome 21 open reading frame 83; PFM15; PR domain containing 15; PR domain zinc finger protein 15; PR domain-containing protein 15; Zinc finger protein 298; ZNF298 PRDM15 C21orf83, PFM15, ZNF298 PR/SET domain 15 PR domain zinc finger protein 15|PR domain 15|PR domain containing 15|zinc finger protein 298
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PRDM15, check out the PRDM15 Infographic
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In this infographic, you will see the following information for PRDM15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PRDM15 (NM_022115) Human Over-expression Lysate (LS063977)
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No publications found for LS063977
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