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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
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Protocols, optimization tips,troubleshooting guides, and more for Flow Cytometry.
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One of the first things to consider when designing your flow cytometry experiment is which controls to use. There are many sources for error and variability that can be introduced into experimental measurements, and it is important to control those points. The purposes of these controls are to ensure the flow cytometry instrument is functioning properly, that compensation is correctly set, that appropriate gates can be drawn, and the correct interpretation of the data can be obtained.
Use this table to help decide which controls are appropriate for your flow cytometry experiment:
Keywords: Flow cytometry, FACS experimental controls, negative and positive control, isotype, FMO, compensation, viability, Fc blocking