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In order for an ELISA to produce useful data, the proteins that become bound in each well must be detected in such a way that the signal strength correlates linearly to the protein concentration. Toward this end, labelled secondary antibodies are used. The choice of which detection system to use is influenced by the equipment available and the needs of the specific application being considered. Use this guide to help decide whether to use enzyme-linked secondary antibodies, or fluorescently labelled secondary antibodies as your detection system.
|Enzyme Conjugates||Fluorescent Conjugates|
Keywords: ELISA, optimization, enzyme, fluorescent, enzyme, conjugate, secondary antibodiesClick for more optimization tips