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Product Info Summary
| SKU: | LS005699 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of proteasome (prosome, macropain) subunit, beta type, 10 (PSMB10) |
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Product info
Product Name
PSMB10 (NM_002801) Human Over-expression Lysate
SKU/Catalog Number
LS005699
Size
100 µg
Description
Transient overexpression lysate of proteasome (prosome, macropain) subunit, beta type, 10 (PSMB10)
Storage & Handling
Cite This Product
PSMB10 (NM_002801) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005699)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PSMB10 Human Over-expression Lysates NM_002801.
Western blot validation of overexpression lysate (LS005699) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PSMB10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PSMB10 (Source: Uniprot.org, NCBI)
Gene Name
PSMB10
Full Name
Proteasome subunit beta type-10
Weight
28.936kDa
Superfamily
peptidase T1B family
Alternative Names
beta2i; FLJ00366; LMP10; LMP10Proteasome subunit beta-2i; Low molecular mass protein 10; Macropain subunit MECl-1; MECL1; MECl-1; MECL1EC 3.4.25.1; MGC1665; Multicatalytic endopeptidase complex subunit MECl-1; proteasome (prosome, macropain) subunit, beta type, 10; proteasome catalytic subunit 2i; Proteasome MECl-1; proteasome subunit beta 7i; proteasome subunit beta type-10; Proteasome subunit beta-2i; proteasome subunit MECL1; PSMB10 PSMB10 LMP10, MECL1, PRAAS5, beta2i proteasome 20S subunit beta 10 proteasome subunit beta type-10|low molecular mass protein 10|macropain subunit MECl-1|multicatalytic endopeptidase complex subunit MECl-1|proteasome (prosome, macropain) subunit, beta type, 10|proteasome MECl-1|proteasome catalytic subunit 2i|proteasome subunit MECL1|proteasome subunit beta 10|proteasome subunit beta 7i|proteasome subunit beta-2i|proteasome subunit beta2i
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PSMB10, check out the PSMB10 Infographic
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In this infographic, you will see the following information for PSMB10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PSMB10 (NM_002801) Human Over-expression Lysate (LS005699)
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