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Product Info Summary
| SKU: | LS005705 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5) |
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Product info
Product Name
PSMC5 (NM_002805) Human Over-expression Lysate
SKU/Catalog Number
LS005705
Size
100 µg
Description
Transient overexpression lysate of proteasome (prosome, macropain) 26S subunit, ATPase, 5 (PSMC5)
Storage & Handling
Cite This Product
PSMC5 (NM_002805) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005705)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PSMC5 Human Over-expression Lysates NM_002805.
Western blot validation of overexpression lysate (LS005705) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PSMC5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for PSMC5 (Source: Uniprot.org, NCBI)
Gene Name
PSMC5
Full Name
26S proteasome regulatory subunit 8
Weight
45.626kDa
Superfamily
AAA ATPase family
Alternative Names
MSUG1 protein; p45; p45/SUG26S protease regulatory subunit 8; proteasome (prosome, macropain) 26S subunit, ATPase, 5; proteasome 26S ATPase subunit 5; Proteasome 26S subunit ATPase 5; Proteasome subunit p45; S8; SUG-1; SUG126S proteasome AAA-ATPase subunit RPT6; Tat-binding protein homolog 10; TBP10; thyroid receptor interactor 1; TRIP1Thyroid hormone receptor-interacting protein 1 PSMC5 RPT6, S8, SUG-1, SUG1, TBP10, TRIP1, p45, p45/SUG proteasome 26S subunit, ATPase 5 26S proteasome regulatory subunit 8|26S protease regulatory subunit 8|26S proteasome AAA-ATPase subunit RPT6|MSUG1 protein|Tat-binding protein homolog 10|proteasome (prosome, macropain) 26S subunit, ATPase, 5|proteasome 26S ATPase subunit 5|proteasome subunit p45|testicular tissue protein Li 149|thyroid hormone receptor-interacting protein 1|thyroid receptor interactor 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on PSMC5, check out the PSMC5 Infographic
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In this infographic, you will see the following information for PSMC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PSMC5 (NM_002805) Human Over-expression Lysate (LS005705)
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