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Product Info Summary
| SKU: | LS005818 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of poliovirus receptor-related 1 (herpesvirus entry mediator C) (PVRL1), transcript variant 3 |
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Product info
Product Name
PVRL1 (NECTIN1) (NM_203286) Human Over-expression Lysate
See all Nectin-1/PVRL1 products
SKU/Catalog Number
LS005818
Size
100 µg
Description
Transient overexpression lysate of poliovirus receptor-related 1 (herpesvirus entry mediator C) (PVRL1), transcript variant 3
Storage & Handling
Cite This Product
PVRL1 (NECTIN1) (NM_203286) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005818)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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PVRL1 (NECTIN1) Human Over-expression Lysates NM_203286.
Western blot validation of overexpression lysate (LS005818) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with NECTIN1 (Myc-DDK-tagged) Human nectin cell adhesion molecule 1 (NECTIN1)
Protein Target Info & Infographic
Gene/Protein Information for NECTIN1 (Source: Uniprot.org, NCBI)
Gene Name
NECTIN1
Full Name
Nectin-1
Weight
57.158kDa
Superfamily
nectin family
Alternative Names
CD111 antigen; CD111; CLPED1ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgRPVRR; HVEC; HVECpoliovirus receptor-like 1; MGC142031; Nectin1; Nectin-1; OFC7nectin 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; PVRR1nectin-1; SK-12 NECTIN1 CD111, CLPED1, ED4, HIgR, HV1S, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR, PVRR1, SK-12, nectin-1 nectin cell adhesion molecule 1 nectin-1|ectodermal dysplasia 4 (Margarita Island type)|herpes simplex virus type 1 sensitivity|herpes virus entry mediator C|herpesvirus Ig-like receptor|nectin 1|poliovirus receptor-like 1|poliovirus receptor-related 1 (herpesvirus entry mediator C)|poliovirus receptor-related protein 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on NECTIN1, check out the NECTIN1 Infographic
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In this infographic, you will see the following information for NECTIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PVRL1 (NECTIN1) (NM_203286) Human Over-expression Lysate (LS005818)
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