RAB8B (NM_016530) Human Over-expression Lysate

Lysate of RAB8B over-expression cell lines, WB control

Transient overexpression lysate of RAB8B, member RAS oncogene family (RAB8B)

Product Info Summary

SKU: LS051762
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of RAB8B, member RAS oncogene family (RAB8B)

Product Name

RAB8B (NM_016530) Human Over-expression Lysate

See all RAB8B products

SKU/Catalog Number

LS051762

Size

100 µg

Description

Transient overexpression lysate of RAB8B, member RAS oncogene family (RAB8B)

Storage & Handling

Cite This Product

RAB8B (NM_016530) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS051762)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for RAB8B (Source: Uniprot.org, NCBI)

Gene Name

RAB8B

Full Name

Ras-related protein Rab-8B

Weight

23.584kDa

Superfamily

small GTPase superfamily

Alternative Names

FLJ38125; RAB-8b protein; RAB8B, member RAS oncogene family; ras-related protein Rab-8B RAB8B RAB8B, member RAS oncogene family ras-related protein Rab-8B|RAB-8b protein

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on RAB8B, check out the RAB8B Infographic

RAB8B infographic

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In this infographic, you will see the following information for RAB8B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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