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Product Info Summary
| SKU: | LS008480 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1 |
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Product info
Product Name
RAE1 (NM_003610) Human Over-expression Lysate
SKU/Catalog Number
LS008480
Size
100 µg
Description
Transient overexpression lysate of RAE1 RNA export 1 homolog (S. pombe) (RAE1), transcript variant 1
Storage & Handling
Cite This Product
RAE1 (NM_003610) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS008480)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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RAE1 Human Over-expression Lysates NM_001015885.
Western blot validation of overexpression lysate (LS008480) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RAE1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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RAE1 Human Over-expression Lysates NM_003610.
Western blot validation of overexpression lysate (LS008480) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RAE1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for RAE1 (Source: Uniprot.org, NCBI)
Gene Name
RAE1
Full Name
mRNA export factor
Weight
40.968kDa
Superfamily
WD repeat rae1 family
Alternative Names
dJ481F12.3; dJ800J21.1; FLJ30608; homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer); MGC117333; MGC126076; MGC126077; MIG14; migration-inducing gene 14; Mnrp41; mRNA export factor; mRNA export protein; mRNA-associated protein mrnp 41; mRNA-binding protein, 41-kD; MRNP41; RAE1 (RNA export 1, S.pombe) homolog; Rae1 protein homolog; RAE1 RNA export 1 homolog (S. pombe) RAE1 Gle2, MIG14, MRNP41, Mnrp41, dJ481F12.3, dJ800J21.1 ribonucleic acid export 1 mRNA export factor|RAE1 RNA export 1 homolog|homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer)|mRNA export protein|mRNA-associated protein MRNP 41|mRNA-binding protein, 41-kD|migration-inducing gene 14|rae1 protein homolog
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on RAE1, check out the RAE1 Infographic
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In this infographic, you will see the following information for RAE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For RAE1 (NM_003610) Human Over-expression Lysate (LS008480)
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