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Product Info Summary
| SKU: | EK1611 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat BMP-9 ELISA Kit PicoKine®
SKU/Catalog Number
EK1611
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat BMP-9 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Gdf2 in bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat BMP-9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1611)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: S320-R430
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1611 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1611 is reactive to GDF2 in Rat samples
Validated Sample Types
bone tissue, cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1611 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BMP-9
Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1611-CAP | Anti-Rat GDF2 Pre-coated 96-well strip microplate | 1 |
| EK1611-ST | Rat GDF2 Standard | 2 vials, 10 ng/tube |
| EK1611-DA | Rat GDF2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.053 | 0.121 | 0.149 | 0.209 | 0.385 | 0.749 | 1.406 | 2.096 |
Data Example Images
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Rat BMP-9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BMP-9, Dilution ratio of 1:1, concentration in serum and plasma is around 120 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat BMP-9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 36 | 187 | 414 | 35 | 192 | 435 |
| Standard deviation | 2.12 | 11.78 | 23.59 | 2.69 | 13.63 | 27.16 |
| CV (%) | 5.7% | 7.1% | 5.7% | 7.7% | 7.1% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BMP-9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 36 | 36 | 38 | 36 | 0.86 | 2.3% |
| Sample 2 | 187 | 182 | 199 | 172 | 185 | 9.72 | 5.2% |
| Sample 3 | 414 | 464 | 457 | 446 | 445 | 19.14 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For GDF2 (Source: Uniprot.Org, NCBI)
Gene Name
GDF2
Full Name
Growth/differentiation factor 2
Weight
47.32kDa
Superfamily
TGF-beta family
Alternative Names
BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2 GDF2 BMP-9, BMP9, HHT5 growth differentiation factor 2 growth/differentiation factor 2|bone morphogenetic protein 9
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GDF2, check out the GDF2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GDF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat BMP-9 ELISA Kit PicoKine® (EK1611)
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
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6 Customer Q&As for Rat BMP-9 ELISA Kit PicoKine®
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
P. Tiwari
Verified customer
Asked: 2020-02-09
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-02-09
Question
Does EK1611 kit contain any recombinant protein except the Rat Gdf2 Standard?
Verified customer
Asked: 2019-12-24
Answer
Yes, the Rat Gdf2 Standard is the only recombinant protein contained in the Rat BMP-9 ELISA Kit PicoKine® (EK1611). Expression system for standard: CHO, Immunogen sequence: S320-R430.
Boster Scientific Support
Answered: 2019-12-30
Question
Q: What is the optimal O.D. value for BMP-9 ELISA kit? I used your BMP-9 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain BMP-9 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-05-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BMP-9 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of BMP-9 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-05-05
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification BMP-9 using the EK1611 Rat BMP-9 Picokine® ELISA Kit?
S. Singh
Verified customer
Asked: 2018-02-15
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-02-15
Question
Q: can I use citrate plasma as samples in Rat BMP-9 Picokine® ELISA Kit (Catalog # EK1611)?
Verified Customer
Verified customer
Asked: 2016-05-27
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of BMP-9 causing degradation of its protein structure. BMP-9 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the BMP-9 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-05-27
Question
Q: how to thaw whole blood sample for BMP-9 ELISA after freezing?
M. Mangal
Verified customer
Asked: 2016-01-27
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP-9 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2016-01-27
