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Product Info Summary
| SKU: | A03443-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-BMP-9 GDF2 Antibody
SKU/Catalog Number
A03443-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-BMP-9 GDF2 Antibody catalog # A03443-1. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMP-9 GDF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03443-1)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 371-420.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03443-1 is reactive to GDF2 in Human, Mouse
Applications
A03443-1 is guaranteed for ELISA, IHC Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
47.32kDa
Background of BMP-9
SERPINB2 is also known as PAI or PAI2. Plasminogen activator inhibitor-2 (placental PAI) is acoagulation factor that inactivates tPA and urokinase. It is present in most cells, especially monocytes/macrophages. PAI-2 exists in two forms, a 60-kDa extracellular glycosylated form and a 43-kDa intracellular form. It is present only at detectable quantities in blood during pregnancy, as it is produced by the placenta, and may explain partially the increased rate of thrombosisduring pregnancy. The majority of expressed PAI-2 remains unsecreted due to the presence of an inefficient internal signal peptide.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 1:50-300
ELISA 1:10000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For GDF2 (Source: Uniprot.org, NCBI)
Gene Name
GDF2
Full Name
Growth/differentiation factor 2
Weight
47.32kDa
Superfamily
TGF-beta family
Alternative Names
BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2 GDF2 BMP-9, BMP9, HHT5 growth differentiation factor 2 growth/differentiation factor 2|bone morphogenetic protein 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GDF2, check out the GDF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GDF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BMP-9 GDF2 Antibody (A03443-1)
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No publications found for A03443-1
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-BMP-9 GDF2 Antibody
Question
I was wanting to use your anti-BMP-9 antibody for IHC for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
As indicated on the product datasheet, A03443-1 anti-BMP-9 antibody has been validated for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-24
Question
Is a blocking peptide available for product anti-BMP-9 antibody (A03443-1)?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We do provide the blocking peptide for product anti-BMP-9 antibody (A03443-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-11
Question
My question regarding product A03443-1, anti-BMP-9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03443-1 anti-BMP-9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-13
Question
Does A03443-1 anti-BMP-9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Edwards
Verified customer
Asked: 2019-06-10
Answer
As indicated on the product datasheet, A03443-1 anti-BMP-9 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-BMP-9 antibody A03443-1. Let me know if you need anything else.
S. Parker
Verified customer
Asked: 2017-06-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-30
Question
We are currently using anti-BMP-9 antibody A03443-1 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
C. Anderson
Verified customer
Asked: 2015-05-06
Answer
The anti-BMP-9 antibody (A03443-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-06
