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Product Info Summary
| SKU: | EK1855 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat PDGF-CC ELISA Kit PicoKine®
SKU/Catalog Number
EK1855
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat PDGF-CC ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Pdgfc in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat PDGF-CC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1855)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A235-G345
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1855 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1855 is reactive to Pdgfc in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1855 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PDGF-C
Platelet-derived growth factor C, also known as PDGF-C, is a 345-amino acid protein that in humans is encoded by the PDGFC gene. It is mapped to 2q33-q34. Platelet-derived growth factors are important in connective tissue growth, survival and function, and consist of disulphide-linked dimers involving two polypeptide chains, PDGF-A and PDGF-B. PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique two-domain structure and expression pattern. PDGF-C was not previously identified with PDGF-A and PDGF-B, possibly because it may be that it is synthesized and secreted as a latent growth factor, requiring proteolytic removal of the N-terminal CUB domain for receptor binding and activation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1855-CAP | Anti-Rat Pdgfc Pre-coated 96-well strip microplate | 1 |
| EK1855-ST | Rat Pdgfc Standard | 2 vials, 10 ng/tube |
| EK1855-DA | Rat Pdgfc Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.032 | 0.085 | 0.143 | 0.248 | 0.423 | 0.816 | 1.359 | 2.031 |
Data Example Images
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Rat PDGF-CC PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PDGF-C, Dilution ratio of 1:1, concentration in serum and plasma is around 30 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat PDGF-CC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 16 | 113 | 464 | 23 | 117 | 472 |
| Standard deviation | 0.64 | 5.31 | 24.13 | 1.17 | 6.90 | 33.51 |
| CV (%) | 4.0% | 4.7% | 5.2% | 5.1% | 5.9% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PDGF-C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 16 | 16 | 19 | 18 | 17 | 1.29 | 7.5% |
| Sample 2 | 113 | 113 | 110 | 122 | 114 | 4.50 | 3.9% |
| Sample 3 | 464 | 458 | 474 | 460 | 464 | 6.16 | 1.3% |
Protein Target Info & Infographic
Gene/Protein Information For Pdgfc (Source: Uniprot.Org, NCBI)
Gene Name
Pdgfc
Full Name
Platelet-derived growth factor C
Weight
38.741kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
FALLOTEIN; PDGFC; PDGF-C; platelet derived growth factor C; platelet-derived growth factor C; SCDGFSpinal cord-derived growth factor; secretory growth factor-like protein; VEGF-E Pdgfc|1110064L01Rik, AI647969, PDGF-, PDGF-C, SCDGF, VEGF-E|platelet-derived growth factor, C polypeptide|platelet-derived growth factor C|fallotein|spinal cord-derived growth factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Pdgfc, check out the Pdgfc Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Pdgfc: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat PDGF-CC ELISA Kit PicoKine® (EK1855)
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No publications found for EK1855
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Rat PDGF-CC ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for PDGF-C ELISA kit? I used your PDGF-C ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain PDGF-C even though the O.D. values are not very high?
N. Gonzalez
Verified customer
Asked: 2019-05-10
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PDGF-C ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of PDGF-C that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-05-10
Question
Q: which procedure should I follow in order to thaw whole blood sample for PDGF-C ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-09-20
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PDGF-C for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-09-20
Question
Q: Can PDGF-C ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Mehta
Verified customer
Asked: 2018-08-23
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-08-23
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification PDGF-C using the EK1855 Rat PDGF-C Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-07-08
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2016-07-08
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-06-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-06-05
