Anti-TRIM71 Antibody

Product Name

Anti-TRIM71 Antibody

View all TRIM71 Antibodies

SKU/Catalog Number

A08005

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TRIM71 Antibody (Catalog # A08005). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TRIM71 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08005)

Host

Rabbit

Contents

TRIM71 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TRIM71 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71. The immunogen is located within amino acids 680 - 730 of TRIM71.

Reactive Species

A08005 is reactive to TRIM71 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

93.4 kDa

Background of TRIM71

TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A08005 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TRIM71 antibody can be used for detection of TRIM71 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TRIM71

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis