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Product Info Summary
| SKU: | LS005439 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa (POLR2J) |
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Product info
Product Name
RPB11 (POLR2J) (NM_006234) Human Over-expression Lysate
SKU/Catalog Number
LS005439
Size
100 µg
Description
Transient overexpression lysate of polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa (POLR2J)
Storage & Handling
Cite This Product
RPB11 (POLR2J) (NM_006234) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005439)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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RPB11 (POLR2J) Human Over-expression Lysates NM_006234.
Western blot validation of overexpression lysate (LS005439) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with POLR2J (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for POLR2J (Source: Uniprot.org, NCBI)
Gene Name
POLR2J
Full Name
DNA-directed RNA polymerase II subunit RPB11-a
Weight
13.293kDa
Superfamily
archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family
Alternative Names
DNA-directed RNA polymerase II subunit J-1; DNA-directed RNA polymerase II subunit RPB11-a; hRPB14; MGC71910; POLR2J1RNA polymerase II subunit B11-a; polymerase (RNA) II (DNA directed) polypeptide J (13.3kD); polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa; RNA polymerase II 13.3 kDa subunit; RPB11; RPB11A; RPB11m POLR2J POLR2J1, RPB11, RPB11A, RPB11m, hRPB14 RNA polymerase II subunit J DNA-directed RNA polymerase II subunit RPB11-a|DNA-directed RNA polymerase II subunit J-1|RNA polymerase II 13.3 kDa subunit|RNA polymerase II subunit B11-a|polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa|polymerase (RNA) II subunit J
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on POLR2J, check out the POLR2J Infographic
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In this infographic, you will see the following information for POLR2J: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For RPB11 (POLR2J) (NM_006234) Human Over-expression Lysate (LS005439)
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