Product Info Summary
SKU: | LS009092 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of squamous cell carcinoma antigen recognized by T cells (SART1) |
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Product info
Product Name
SART1 (NM_005146) Human Over-expression Lysate
SKU/Catalog Number
LS009092
Size
100 µg
Description
Transient overexpression lysate of squamous cell carcinoma antigen recognized by T cells (SART1)
Storage & Handling
Cite This Product
SART1 (NM_005146) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009092)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
SART1 Human Over-expression Lysates NM_005146.
Western blot validation of overexpression lysate (LS009092) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SART1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SART1 (Source: Uniprot.org, NCBI)
Gene Name
SART1
Full Name
U4/U6.U5 tri-snRNP-associated protein 1
Weight
90.255kDa
Superfamily
SNU66/SART1 family
Alternative Names
Allergen Hom s 1; Ara1; HAF; HOMS1; hSART-1; hSnu66; hypoxia-associated factor; IgE Autoantigen; MGC2038; SART1; SART-1; SART1(259) protein; SART1(800) protein; SART1259; small nuclear ribonucleoprotein 110kDa (U4/U6.U5); SNRNP110; SNU66 homolog; Snu66; squamous cell carcinoma antigen recognised by T cells; Squamous cell carcinoma antigen recognized by T cells 1; squamous cell carcinoma antigen recognized by T cells; U4/U6.U5 tri-snRNP-associated 110 kDa protein; U4/U6.U5 tri-snRNP-associated protein 1 SART1 Ara1, HAF, HOMS1259, SNRNP110, Snu66, SART1 spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP U4/U6.U5 tri-snRNP-associated protein 1|IgE autoantigen|SART-1|SART1(259) protein|SART1(800) protein|SART1, U4/U6.U5 tri-snRNP-associated protein 1|SNU66 homolog|U4/U6.U5 tri-snRNP-associated 110 kDa protein|hSART-1|hSnu66|hypoxia associated factor|small nuclear ribonucleoprotein 110kDa (U4/U6.U5)|squamous cell carcinoma antigen recognised by T cells|squamous cell carcinoma antigen recognized by T-cells 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SART1, check out the SART1 Infographic
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In this infographic, you will see the following information for SART1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SART1 (NM_005146) Human Over-expression Lysate (LS009092)
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