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Product Info Summary
| SKU: | LS004068 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of SH2 domain protein 1A (SH2D1A), transcript variant 1 |
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Product info
Product Name
SH2D1A (NM_002351) Human Over-expression Lysate
SKU/Catalog Number
LS004068
Size
100 µg
Description
Transient overexpression lysate of SH2 domain protein 1A (SH2D1A), transcript variant 1
Storage & Handling
Cite This Product
SH2D1A (NM_002351) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS004068)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SH2D1A Human Over-expression Lysates NM_002351.
Western blot validation of overexpression lysate (LS004068) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SH2D1A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SH2D1A (Source: Uniprot.org, NCBI)
Gene Name
SH2D1A
Full Name
SH2 domain-containing protein 1A
Weight
14.187kDa
Alternative Names
DSHP; DSHPLYP; Duncan disease SH2-protein; EBVS; FLJ92177; IMD5; LYP; MTCP1; SAP/SH2D1A; SAPlymphoproliferative syndrome; SH2 domain containing 1A; SH2 domain-containing protein 1A; SH2D1A; signaling lymphocyte activation molecule-associated protein; Signaling lymphocytic activation molecule-associated protein; SLAM associated protein/SH2 domain protein 1A; SLAM-associated protein; T cell signal transduction molecule SAP; T-cell signal transduction molecule SAP; XLP; XLPD; XLPDFLJ18687; XLPSH2 domain protein 1A SH2D1A DSHP, EBVS, IMD5, LYP, MTCP1, SAP, SAP/SH2D1A, XLP, XLPD, XLPD1 SH2 domain containing 1A SH2 domain-containing protein 1A|Duncan disease SH2-protein|SLAM associated protein/SH2 domain protein 1A|SLAM-associated protein|T cell signal transduction molecule SAP|signaling lymphocyte activation molecule-associated protein|signaling lymphocytic activation molecule-associated protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SH2D1A, check out the SH2D1A Infographic
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In this infographic, you will see the following information for SH2D1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SH2D1A (NM_002351) Human Over-expression Lysate (LS004068)
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