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Product Info Summary
| SKU: | LS054716 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of solute carrier family 6 (proline IMINO transporter), member 20 (SLC6A20), transcript variant 1 |
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Product info
Product Name
SLC6A20 (NM_020208) Human Over-expression Lysate
SKU/Catalog Number
LS054716
Size
100 µg
Description
Transient overexpression lysate of solute carrier family 6 (proline IMINO transporter), member 20 (SLC6A20), transcript variant 1
Storage & Handling
Cite This Product
SLC6A20 (NM_020208) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS054716)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SLC6A20 Human Over-expression Lysates NM_020208.
Western blot validation of overexpression lysate (LS054716) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC6A20 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SLC6A20 (Source: Uniprot.org, NCBI)
Gene Name
SLC6A20
Full Name
Sodium- and chloride-dependent transporter XTRP3
Weight
65.914kDa
Superfamily
sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family
Alternative Names
MGC161475; neurotransmitter transporter RB21A; SIT1; sodium- and chloride-dependent transporter XTRP3; Sodium/imino-acid transporter 1; solute carrier family 6 (neurotransmitter transporter), member 20; solute carrier family 6 (proline IMINO transporter), member 20; Solute carrier family 6 member 20; Transporter rB21A homolog; X transporter protein 3; XT3orphan transporter XT3; Xtrp3 SLC6A20 IMINO, SIT1, XT3, Xtrp3 solute carrier family 6 member 20 sodium- and chloride-dependent transporter XTRP3|X transporter protein 3|neurotransmitter transporter RB21A|orphan transporter XT3|sodium-dependent imino acid transporter 1|sodium/imino-acid transporter 1|solute carrier family 6 (neurotransmitter transporter), member 20|solute carrier family 6 (proline IMINO transporter), member 20|transporter rB21A homolog
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SLC6A20, check out the SLC6A20 Infographic
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In this infographic, you will see the following information for SLC6A20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SLC6A20 (NM_020208) Human Over-expression Lysate (LS054716)
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