SLITRK5 (NM_015567) Human Over-expression Lysate

Lysate of SLITRK5 over-expression cell lines, WB control

Transient overexpression lysate of SLIT and NTRK-like family, member 5 (SLITRK5)

Product Info Summary

SKU: LS026050
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of SLIT and NTRK-like family, member 5 (SLITRK5)

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Product Name

SLITRK5 (NM_015567) Human Over-expression Lysate

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SKU/Catalog Number

LS026050

Size

100 µg

Description

Transient overexpression lysate of SLIT and NTRK-like family, member 5 (SLITRK5)

Storage & Handling

Cite This Product

SLITRK5 (NM_015567) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS026050)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SLITRK5 (Source: Uniprot.org, NCBI)

Gene Name

SLITRK5

Full Name

SLIT and NTRK-like protein 5

Weight

107.486kDa

Superfamily

SLITRK family

Alternative Names

bA364G4.2; FLJ58374; KIAA0918; leucine-rich repeat-containing protein 11; LRRC11; SLIT and NTRK-like family, member 5; SLIT and NTRK-like protein 5; SLITRK5 SLITRK5 LRRC11, bA364G4.2 SLIT and NTRK like family member 5 SLIT and NTRK-like protein 5|leucine rich repeat containing 11|leucine-rich repeat-containing protein 11|slit and trk like gene 5

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on SLITRK5, check out the SLITRK5 Infographic

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