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Product Info Summary
| SKU: | LS006342 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of sterol carrier protein 2 (SCP2), transcript variant 1 |
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Product info
Product Name
Sterol carrier protein 2 (SCP2) (NM_002979) Human Over-expression Lysate
See all Sterol carrier protein 2 products
SKU/Catalog Number
LS006342
Size
100 µg
Description
Transient overexpression lysate of sterol carrier protein 2 (SCP2), transcript variant 1
Storage & Handling
Cite This Product
Sterol carrier protein 2 (SCP2) (NM_002979) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006342)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Sterol carrier protein 2 (SCP2) Human Over-expression Lysates NM_001007099.
Western blot validation of overexpression lysate (LS006342) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SCP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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Sterol carrier protein 2 (SCP2) Human Over-expression Lysates NM_001007100.
Western blot validation of overexpression lysate (LS006342) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SCP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
Sterol carrier protein 2 (SCP2) Human Over-expression Lysates NM_001193599.
Western blot validation of overexpression lysate (LS006342) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SCP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
Sterol carrier protein 2 (SCP2) Human Over-expression Lysates NM_001193600.
Western blot validation of overexpression lysate (LS006342) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SCP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
Sterol carrier protein 2 (SCP2) Human Over-expression Lysates NM_001193617.
Western blot validation of overexpression lysate (LS006342) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SCP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
Sterol carrier protein 2 (SCP2) Human Over-expression Lysates NM_002979.
Western blot validation of overexpression lysate (LS006342) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SCP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SCP2 (Source: Uniprot.org, NCBI)
Gene Name
SCP2
Full Name
Non-specific lipid-transfer protein
Weight
58.994kDa
Alternative Names
DKFZp686C12188; DKFZp686D11188; NLTP; non-specific lipid-transfer protein; NSL-TP; Propanoyl-CoA C-acyltransferase; SCP-2; SCP-CHI; SCPX; SCP-X; sterol carrier protein 2EC 2.3.1.176; Sterol carrier protein X SCP2 NLTP, NSL-TP, SCOX, SCP-2, SCP-CHI, SCP-X, SCPX sterol carrier protein 2 sterol carrier protein 2|SCP-2/3-oxoacyl-CoA thiolase|SCP-2/thiolase|acetyl-CoA C-myristoyltransferase|epididymis secretory sperm binding protein|non-specific lipid-transfer protein|propanoyl-CoA C-acyltransferase|sterol carrier protein X|straight-chain acyl-CoA oxidase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SCP2, check out the SCP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Sterol carrier protein 2 (SCP2) (NM_002979) Human Over-expression Lysate (LS006342)
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