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Product Info Summary
| SKU: | LS004070 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of tumor-associated calcium signal transducer 2 (TACSTD2) |
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Product info
Product Name
TACD2 (TACSTD2) (NM_002353) Human Over-expression Lysate
SKU/Catalog Number
LS004070
Size
100 µg
Description
Transient overexpression lysate of tumor-associated calcium signal transducer 2 (TACSTD2)
Storage & Handling
Cite This Product
TACD2 (TACSTD2) (NM_002353) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS004070)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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TACD2 (TACSTD2) Human Over-expression Lysates NM_002353.
Western blot validation of overexpression lysate (LS004070) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TACSTD2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TACSTD2 (Source: Uniprot.org, NCBI)
Gene Name
TACSTD2
Full Name
Tumor-associated calcium signal transducer 2
Weight
35.709kDa
Superfamily
EPCAM family
Alternative Names
Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2 TACSTD2 EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2 tumor associated calcium signal transducer 2 tumor-associated calcium signal transducer 2|40kD glycoprotein, identified by monoclonal antibody GA733|cell surface glycoprotein TROP2|cell surface glycoprotein Trop-2|epithelial glycoprotein-1|gastrointestinal tumor-associated antigen GA7331|membrane component, chromosome 1, surface marker 1|pancreatic carcinoma marker protein GA733-1|pancreatic carcinoma marker protein GA7331|trophoblast cell surface antigen 2|truncated TACSTD2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TACSTD2, check out the TACSTD2 Infographic
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In this infographic, you will see the following information for TACSTD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TACD2 (TACSTD2) (NM_002353) Human Over-expression Lysate (LS004070)
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