TAF2 (NM_003184) Human Over-expression Lysate

Product Name

TAF2 (NM_003184) Human Over-expression Lysate

See all TAF2 products

SKU/Catalog Number

LS006873

Size

100 µg

Description

Transient overexpression lysate of TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (TAF2)

Storage & Handling

Cite This Product

TAF2 (NM_003184) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006873)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for TAF2 (Source: Uniprot.org, NCBI)

Alternative Names

150 kDa cofactor of initiator function; CIF150TAFII-150; cofactor of initiator function, 150kD subunit; TAF(II)150; TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor; TAF2B; TAFII150RNA polymerase II TBP-associated factor subunit B; TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD; TBP-associated factor 150 kDa; Transcription initiation factor TFIID 150 kDa subunit; transcription initiation factor TFIID subunit 2 TAF2 CIF150, MRT40B, TAFII150, TAF2 TATA-box binding protein associated factor 2 transcription initiation factor TFIID subunit 2|150 kDa cofactor of initiator function|RNA polymerase II TBP-associated factor subunit B|TAF(II)150|TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa|TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD|TBP-associated factor 150 kDa|cofactor of initiator function, 150kD subunit|transcription initiation factor TFIID 150 kDa subunit

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful:

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis