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Product Info Summary
| SKU: | LS090665 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of transducin (beta)-like 1, Y-linked (TBL1Y), transcript variant 1 |
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Product info
Product Name
TBL1Y (NM_033284) Human Over-expression Lysate
SKU/Catalog Number
LS090665
Size
100 µg
Description
Transient overexpression lysate of transducin (beta)-like 1, Y-linked (TBL1Y), transcript variant 1
Storage & Handling
Cite This Product
TBL1Y (NM_033284) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS090665)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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TBL1Y Human Over-expression Lysates NM_033284.
Western blot validation of overexpression lysate (LS090665) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TBL1Y (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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TBL1Y Human Over-expression Lysates NM_134258.
Western blot validation of overexpression lysate (LS090665) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TBL1Y (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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TBL1Y Human Over-expression Lysates NM_134259.
Western blot validation of overexpression lysate (LS090665) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TBL1Y (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TBL1Y (Source: Uniprot.org, NCBI)
Gene Name
TBL1Y
Full Name
F-box-like/WD repeat-containing protein TBL1Y
Weight
56.688kDa
Superfamily
WD repeat EBI family
Alternative Names
F-box-like/WD repeat-containing protein TBL1Y; TBL1; TBL1Y transducin (beta)-like 1, Y-linked; transducin beta-like 1; transducin beta-like protein 1Y; transducin-beta-like protein 1, Y-linked TBL1Y DFNY2, TBL1 transducin beta like 1 Y-linked F-box-like/WD repeat-containing protein TBL1Y|transducin beta-like 1|transducin beta-like protein 1Y|transducin-beta-like protein 1, Y-linked
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TBL1Y, check out the TBL1Y Infographic
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In this infographic, you will see the following information for TBL1Y: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TBL1Y (NM_033284) Human Over-expression Lysate (LS090665)
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