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Product Info Summary
| SKU: | LS006929 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1 |
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Product info
Product Name
TCF3 / E2A (TCF3) (NM_003200) Human Over-expression Lysate
SKU/Catalog Number
LS006929
Size
100 µg
Description
Transient overexpression lysate of transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) (TCF3), transcript variant 1
Storage & Handling
Cite This Product
TCF3 / E2A (TCF3) (NM_003200) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006929)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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TCF3 / E2A (TCF3) Human Over-expression Lysates NM_001136139.
Western blot validation of overexpression lysate (LS006929) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TCF3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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TCF3 / E2A (TCF3) Human Over-expression Lysates NM_003200.
Western blot validation of overexpression lysate (LS006929) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TCF3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TCF3 (Source: Uniprot.org, NCBI)
Gene Name
TCF3
Full Name
Transcription factor E2-alpha
Weight
67.6kDa
Alternative Names
bHLHb21; bHLHb21TCF-3; Class B basic helix-loop-helix protein 21; E2A immunoglobulin enhancer-binding factor E12/E47; E2A; E2AKappa-E2-binding factor; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; ITF1Transcription factor ITF-1; Kappa-E2-binding factor; MGC129647; MGC129648; Pan2; TCF-3; Tcfe2a; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor E2-alpha; VDIR; VDR interacting repressor TCF3 AGM8, E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, p75 transcription factor 3 transcription factor E2-alpha|NOL1-TCF3 fusion|VDR interacting repressor|class B basic helix-loop-helix protein 21|helix-loop-helix protein HE47|immunoglobulin transcription factor 1|kappa-E2-binding factor|negative vitamin D response element-binding protein|transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)|transcription factor 3 variant 3|transcription factor ITF-1|vitamin D receptor-interacting repressor
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TCF3, check out the TCF3 Infographic
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In this infographic, you will see the following information for TCF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TCF3 / E2A (TCF3) (NM_003200) Human Over-expression Lysate (LS006929)
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