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Product Info Summary
| SKU: | LS010312 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1 |
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Product info
Product Name
TCIRG1 (NM_006019) Human Over-expression Lysate
SKU/Catalog Number
LS010312
Size
100 µg
Description
Transient overexpression lysate of T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1
Storage & Handling
Cite This Product
TCIRG1 (NM_006019) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010312)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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TCIRG1 Human Over-expression Lysates NM_006019.
Western blot validation of overexpression lysate (LS010312) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TCIRG1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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TCIRG1 Human Over-expression Lysates NM_006053.
Western blot validation of overexpression lysate (LS010312) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TCIRG1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TCIRG1 (Source: Uniprot.org, NCBI)
Gene Name
TCIRG1
Full Name
V-type proton ATPase 116 kDa subunit a isoform 3
Weight
92.968kDa
Superfamily
V-ATPase 116 kDa subunit family
Alternative Names
a3; Atp6i; ATP6N1C; ATP6N1Cspecific 116-kDa vacuolar proton pump subunit; ATP6V0A3T-cell immune response cDNA 7; OC-116 kDa; OC116; OC-116; OC-116kDa; OC116Vph1; OPTB1; Osteoclastic proton pump 116 kDa subunit; Stv1; T-cell immune regulator 1; T-cell immune response cDNA7 protein; T-cell, immune regulator 1; T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a; T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3; T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein aisoform 3; T-cell, immune regulator 1, ATPase, H+ transporti TCIRG1 ATP6N1C, ATP6V0A3, Atp6i, OC-116kDa, OC116, OPTB1, Stv1, TIRC7, Vph1, a3 T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 V-type proton ATPase 116 kDa subunit a3|ATPase, H+ transporting, 116kD|T-cell immune response cDNA 7|T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a|T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3|V-ATPase 116 kDa subunit a3|V-ATPase 116-kDa|V-type proton ATPase 116 kDa subunit a|osteoclastic proton pump 116 kDa subunit|specific 116-kDa vacuolar proton pump subunit|vacuolar proton translocating ATPase 116 kDa subunit A
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TCIRG1, check out the TCIRG1 Infographic
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In this infographic, you will see the following information for TCIRG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TCIRG1 (NM_006019) Human Over-expression Lysate (LS010312)
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