TRIM24 Colorimetric Cell-Based ELISA

TIF1 alpha ELISA kit, cell-based

The TRIM24 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TRIM24 protein expression profile in cells. The kit can be used for measuring the relative amounts of TRIM24 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TRIM24.

Product Info Summary

SKU: EKC1627
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

Product Name

TRIM24 Colorimetric Cell-Based ELISA

See all TIF1 alpha products

SKU/Catalog Number

EKC1627

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The TRIM24 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TRIM24 protein expression profile in cells. The kit can be used for measuring the relative amounts of TRIM24 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TRIM24.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

TRIM24 Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1627)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1627 is reactive to TRIM24 in Human, Mouse samples

Application Guarantee

EKC1627 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-TRIM24 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For TRIM24 (Source: Uniprot.Org, NCBI)

Gene Name

TRIM24

Full Name

Transcription intermediary factor 1-alpha

Weight

116.831kDa

Alternative Names

E3 ubiquitin-protein ligase TRIM24; EC 6.3.2; EC 6.3.2.-; hTIF1; PTC6; RING finger protein 82; RNF82Tif1a; TIF1-alpha; TIF1ATIF1ALPHA; TIF1TF1A; transcription intermediary factor 1-alpha; transcriptional intermediary factor 1; tripartite motif containing 24; tripartite motif-containing 24; Tripartite motif-containing protein 24 TRIM24 PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1 tripartite motif containing 24 transcription intermediary factor 1-alpha|E3 ubiquitin-protein ligase TRIM24|RING finger protein 82|RING-type E3 ubiquitin transferase TIF1-alpha|TIF1-alpha|transcriptional intermediary factor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TRIM24, check out the TRIM24 Infographic

TRIM24 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TRIM24: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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