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Product Info Summary
|Description:||Transient overexpression lysate of testis-specific serine kinase 6 (TSSK6)|
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TSSK6 (NM_032037) Human Over-expression Lysate
Transient overexpression lysate of testis-specific serine kinase 6 (TSSK6)
Storage & Handling
Cite This Product
TSSK6 (NM_032037) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083983)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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TSSK6 Human Over-expression Lysates NM_032037.
Western blot validation of overexpression lysate (LS083983) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TSSK6 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for TSSK6 (Source: Uniprot.org, NCBI)
Testis-specific serine/threonine-protein kinase 6
protein kinase superfamily
Cancer/testis antigen 72; CT72TSSK-6; EC 18.104.22.168; FLJ24002; serine/threonine protein kinase SSTK; Serine/threonine-protein kinase SSTK; Small serine/threonine kinase; SSTKTSK-6; Testis-specific kinase 6; testis-specific serine kinase 6; testis-specific serine/threonine-protein kinase 6; TSSK4 TSSK6 CT72, FKSG82, SSTK, TSSK4 testis specific serine kinase 6 testis-specific serine/threonine-protein kinase 6|TSK-6|cancer/testis antigen 72|serine/threonine protein kinase SSTK|small serine/threonine kinase|testis secretory sperm-binding protein Li 205a*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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