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Product Info Summary
| SKU: | LS011066 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of U11/U12 snRNP 35K (U1SNRNPBP), transcript variant 1 |
Product info
Product Name
U1SNRNPBP (SNRNP35) (NM_007020) Human Over-expression Lysate
SKU/Catalog Number
LS011066
Size
100 µg
Description
Transient overexpression lysate of U11/U12 snRNP 35K (U1SNRNPBP), transcript variant 1
Storage & Handling
Cite This Product
U1SNRNPBP (SNRNP35) (NM_007020) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS011066)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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U1SNRNPBP (SNRNP35) Human Over-expression Lysates NM_007020.
Western blot validation of overexpression lysate (LS011066) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with U1SNRNPBP (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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U1SNRNPBP (SNRNP35) Human Over-expression Lysates NM_180699.
Western blot validation of overexpression lysate (LS011066) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SNRNP35 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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U1SNRNPBP (SNRNP35) Human Over-expression Lysates NM_180703.
Western blot validation of overexpression lysate (LS011066) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SNRNP35 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SNRNP35 (Source: Uniprot.org, NCBI)
Gene Name
SNRNP35
Full Name
U11/U12 small nuclear ribonucleoprotein 35 kDa protein
Weight
29.45kDa
Alternative Names
HM1; HM-1; MGC138160; Protein HM-1; small nuclear ribonucleoprotein 35kDa (U11/U12); U1 snRNP-binding protein homolog; U11/U12 small nuclear ribonucleoprotein 35 kDa protein; U11/U12 snRNP 35 kDa protein; U11/U12 snRNP 35K; U11/U12-35K; U1-snRNP binding protein homolog; U1SNRNPBPU1 snRNP binding protein homolog SNRNP35 HM-1, U1SNRNPBP small nuclear ribonucleoprotein U11/U12 subunit 35 U11/U12 small nuclear ribonucleoprotein 35 kDa protein|U1 snRNP-binding protein homolog|U11/U12 snRNP 35 kDa protein|U11/U12 snRNP 35K|U11/U12-35K|protein HM-1|small nuclear ribonucleoprotein 35kDa (U11/U12)|small nuclear ribonucleoprotein, U11/U12 35kDa subunit
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SNRNP35, check out the SNRNP35 Infographic
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In this infographic, you will see the following information for SNRNP35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For U1SNRNPBP (SNRNP35) (NM_007020) Human Over-expression Lysate (LS011066)
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