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Product Info Summary
| SKU: | LS007320 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ubiquitin-conjugating enzyme E2B (RAD6 homolog) (UBE2B) |
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Product info
Product Name
UBE2B (NM_003337) Human Over-expression Lysate
SKU/Catalog Number
LS007320
Size
100 µg
Description
Transient overexpression lysate of ubiquitin-conjugating enzyme E2B (RAD6 homolog) (UBE2B)
Storage & Handling
Cite This Product
UBE2B (NM_003337) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS007320)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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UBE2B Human Over-expression Lysates NM_003337.
Western blot validation of overexpression lysate (LS007320) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with UBE2B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for UBE2B (Source: Uniprot.org, NCBI)
Gene Name
UBE2B
Full Name
Ubiquitin-conjugating enzyme E2 B
Weight
17.312kDa
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
E2 protein; E2-17k; E2-17kDa; HHR6B; HR6B; RAD6 homolog B; RAD6BEC 6.3.2.19; UBC2; UBE2B; Ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2 B; Ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); Ubiquitin-protein ligase B UBE2B E2-17kDa, HHR6B, HR6B, RAD6B, UBC2 ubiquitin conjugating enzyme E2 B ubiquitin-conjugating enzyme E2 B|E2 protein|E2 ubiquitin-conjugating enzyme B|RAD6 homolog B|ubiquitin carrier protein B|ubiquitin conjugating enzyme E2B|ubiquitin-conjugating enzyme E2-17 kDa|ubiquitin-conjugating enzyme E2B (RAD6 homolog)|ubiquitin-protein ligase B
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on UBE2B, check out the UBE2B Infographic
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In this infographic, you will see the following information for UBE2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For UBE2B (NM_003337) Human Over-expression Lysate (LS007320)
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