UBE2CBP (UBE3D) (NM_198920) Human Over-expression Lysate

Product Name

UBE2CBP (UBE3D) (NM_198920) Human Over-expression Lysate

See all UBE2CBP products

SKU/Catalog Number

LS090025

Size

100 µg

Description

Transient overexpression lysate of ubiquitin-conjugating enzyme E2C binding protein (UBE2CBP)

Storage & Handling

Cite This Product

UBE2CBP (UBE3D) (NM_198920) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS090025)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for UBE3D (Source: Uniprot.org, NCBI)

Alternative Names

C6orf157; chromosome 6 open reading frame 157; EC 6.3.2; EC 6.3.2.-; H10BHDKFZp434A1520; MGC126561; MGC126587; UBCH10 binding protein with a hect-like domain; UbcH10-binding protein with a HECT-like domain; ubiquitin-conjugating enzyme E2C binding protein; ubiquitin-conjugating enzyme E2C-binding protein; YJR141W UBE3D C6orf157, H10BH, UBE2CBP, YJR141W ubiquitin protein ligase E3D E3 ubiquitin-protein ligase E3D|HECT-type E3 ubiquitin transferase E3D|UBCH10 binding protein with a hect-like domain|ubcH10-binding protein with a HECT-like domain|ubiquitin-conjugating enzyme E2C-binding protein

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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