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Product Info Summary
| SKU: | LS065109 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B), transcript variant 2 |
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Product info
Product Name
UPF3B (NM_023010) Human Over-expression Lysate
SKU/Catalog Number
LS065109
Size
100 µg
Description
Transient overexpression lysate of UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B), transcript variant 2
Storage & Handling
Cite This Product
UPF3B (NM_023010) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS065109)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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UPF3B Human Over-expression Lysates NM_023010.
Western blot validation of overexpression lysate (LS065109) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with UPF3B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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UPF3B Human Over-expression Lysates NM_080632.
Western blot validation of overexpression lysate (LS065109) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with UPF3B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for UPF3B (Source: Uniprot.org, NCBI)
Gene Name
UPF3B
Full Name
Regulator of nonsense transcripts 3B
Weight
57.762kDa
Superfamily
RENT3 family
Alternative Names
HUPF3B; Nonsense mRNA reducing factor 3B; regulator of nonsense transcripts 3B; RENT3BhUpf3p-X; UPF3 regulator of nonsense transcripts homolog B (yeast); UPF3XMRXS14; Up-frameshift suppressor 3 homolog B; Up-frameshift suppressor 3 homolog on chromosome X UPF3B HUPF3B, MRX62, MRX82, MRXS14, RENT3BP1, UPF3BP2, UPF3BP3, UPF3X, Upf3p-X, UPF3B UPF3B regulator of nonsense mediated mRNA decay regulator of nonsense transcripts 3B|UPF3 regulator of nonsense transcripts homolog B|UPF3B pseudogene 1|UPF3B pseudogene 2|UPF3B pseudogene 3|mental retardation, X-linked 62|nonfunctional UPF3B regulator of nonsense mediated mRNA decay|nonsense mRNA reducing factor 3B|up-frameshift suppressor 3 homolog B|up-frameshift suppressor 3 homolog on chromosome X
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on UPF3B, check out the UPF3B Infographic
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In this infographic, you will see the following information for UPF3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For UPF3B (NM_023010) Human Over-expression Lysate (LS065109)
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