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Product Info Summary
| SKU: | LS158399 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of zinc finger protein 483 (ZNF483), transcript variant 2 |
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Product info
Product Name
ZNF483 (NM_001007169) Human Over-expression Lysate
SKU/Catalog Number
LS158399
Size
100 µg
Description
Transient overexpression lysate of zinc finger protein 483 (ZNF483), transcript variant 2
Storage & Handling
Cite This Product
ZNF483 (NM_001007169) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS158399)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ZNF483 Human Over-expression Lysates NM_001007169.
Western blot validation of overexpression lysate (LS158399) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZNF483 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ZNF483 (Source: Uniprot.org, NCBI)
Gene Name
ZNF483
Full Name
Zinc finger protein 483
Weight
85.098kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
BMZF-2zinc finger 2, bone marrow; BMZF2zinc finger protein 27 (KOX 22); Bone marrow zinc finger 2; KOX22Zinc finger protein 233; zinc finger protein 224; Zinc finger protein 255Zinc finger protein KOX22; Zinc finger protein 27; ZNF255zinc finger protein ZNF255; ZNF27ZNF233 ZNF483 ZKSCAN16, ZSCAN48 zinc finger protein 483 zinc finger protein 483|zinc finger protein HIT-10|zinc finger protein with KRAB and SCAN domains 16
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ZNF483, check out the ZNF483 Infographic
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In this infographic, you will see the following information for ZNF483: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ZNF483 (NM_001007169) Human Over-expression Lysate (LS158399)
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No publications found for LS158399
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