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Product Info Summary
| SKU: | LS064746 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of acyl-Coenzyme A binding domain containing 3 (ACBD3) |
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Product info
Product Name
ACBD3 (NM_022735) Human Over-expression Lysate
SKU/Catalog Number
LS064746
Size
100 µg
Description
Transient overexpression lysate of acyl-Coenzyme A binding domain containing 3 (ACBD3)
Storage & Handling
Cite This Product
ACBD3 (NM_022735) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS064746)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ACBD3 Human Over-expression Lysates NM_022735.
Western blot validation of overexpression lysate (LS064746) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ACBD3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ACBD3 (Source: Uniprot.org, NCBI)
Gene Name
ACBD3
Full Name
Golgi resident protein GCP60
Weight
60.593kDa
Alternative Names
acyl-CoA binding domain containing 3; Acyl-CoA-binding domain-containing protein 3; acyl-Coenzyme A binding domain containing 3; GCP60Golgi phosphoprotein 1; GOCAP1Peripheral benzodiazepine receptor-associated protein PAP7; golgi complex associated protein 1, 60kDa; Golgi complex-associated protein 1; Golgi resident protein GCP60; GOLPH1PBR- and PKA-associated protein 7; PAP7; PBR associated protein; peripherial benzodiazepine receptor associated protein; PKA (RIalpha)-associated protein ACBD3 GCP60, GOCAP1, GOLPH1, PAP7 acyl-CoA binding domain containing 3 Golgi resident protein GCP60|PBR- and PKA-associated protein 7|PKA (RIalpha)-associated protein|acyl-Coenzyme A binding domain containing 3|golgi complex associated protein 1, 60kDa|golgi phosphoprotein 1|peripheral benzodiazepine receptor-associated protein PAP7
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ACBD3, check out the ACBD3 Infographic
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In this infographic, you will see the following information for ACBD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ACBD3 (NM_022735) Human Over-expression Lysate (LS064746)
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No publications found for LS064746
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