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Product Info Summary
| SKU: | A05645 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GOLPH1 ACBD3 Antibody
SKU/Catalog Number
A05645
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GOLPH1 ACBD3 Antibody (Catalog # A05645). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
GOLPH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-GOLPH1 ACBD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05645)
Host
Rabbit
Contents
GOLPH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GOLPH1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human GOLPH1. The immunogen is located within amino acids 50 - 100 of GOLPH1.
Reactive Species
A05645 is reactive to ACBD3 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
60.6 kDa
Background of ACBD3
GOLPH1, also known as GCP60, was initially identified as a Golgi protein that can interact with the integral membrane protein giantin and is thought to be involved in the maintenance of the Golgi structure. GOLPH1 has also been shown to interact with other Golgi proteins such as Golgin-160, a Golgi protein that can be cleaved by caspases-2, -3, and -7, leading to the nuclear localization of Golgin-160. GOLPH1 interaction with the Golgin-160 fragments is stronger than that with the intact Golgin-160, with its interaction regulated by the oxidation state of Cys-463 within GOLPH1, suggesting that the nuclear localization of the caspase-cleaved Golgin-160 fragments is a highly coordinated event. GOLPH1 has also been found to interact with Numb, a cytosolic signaling protein that mediates asymetric cell division of neural progenitor cells to a daughter progenitor cell and a neuron, suggesting that Golgi fragmentation and reconstitution during the cell cycle differentially regulate Numb signaling through changes in GOLPH1 subcellular distribution and may couple cell fate with cell cycle progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05645 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GOLPH1 antibody can be used for detection of GOLPH1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GOLPH1 in K562 cell lysate with GOLPH1 antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-GOLPH1 ACBD3 Antibody (A05645)
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