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Product Info Summary
| SKU: | A05028-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-ACADS/SCAD Antibody Picoband™
SKU/Catalog Number
A05028-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ACADS/SCAD Antibody Picoband™ catalog # A05028-1. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACADS/SCAD Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05028-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ACADS/SCAD, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05028-1 is reactive to ACADS in Human, Mouse, Rat
Applications
A05028-1 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
44 kDa
Calculated molecular weight
44.297kDa
Background of ACADS
Acyl-CoA dehydrogenase, C-2 to C-3 short chain is an enzyme that in humans is encoded by the ACADS gene. This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ACADS/SCAD using anti-ACADS/SCAD antibody (A05028-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Hek293 whole cell lysates,
Lane 6: rat liver tissue lysates,
Lane 7: rat kidney tissue lysates,
Lane 8: mouse liver tissue lysates,
Lane 9: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADS/SCAD antigen affinity purified polyclonal antibody (Catalog # A05028-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACADS/SCAD at approximately 44KD. The expected band size for ACADS/SCAD is at 44KD.
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Figure 2. IF analysis of ACADS/SCAD using anti-ACADS/SCAD antibody (A05028-1).
ACADS/SCAD was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ACADS/SCAD Antibody (A05028-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ACADS (Source: Uniprot.org, NCBI)
Gene Name
ACADS
Full Name
Short-chain specific acyl-CoA dehydrogenase, mitochondrial
Weight
44.297kDa
Superfamily
acyl-CoA dehydrogenase family
Alternative Names
ACAD3; acyl-CoA dehydrogenase, C-2 to C-3 short chain; acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain; Butyryl-CoA dehydrogenase; EC 1.3.99; EC 1.3.99.2; mitochondrial short-chain specific acyl-CoA dehydrogenase; SCADshort-chain acyl-CoA dehydrogenase; short-chain specific acyl-CoA dehydrogenase, mitochondrial; unsaturated acyl-CoA reductase ACADS ACAD3, SCAD acyl-CoA dehydrogenase short chain short-chain specific acyl-CoA dehydrogenase, mitochondrial|acyl-CoA dehydrogenase, C-2 to C-3 short chain|acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain|butyryl-CoA dehydrogenase|epididymis secretory sperm binding protein|mitochondrial short-chain specific acyl-CoA dehydrogenase|unsaturated acyl-CoA reductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACADS, check out the ACADS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACADS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACADS/SCAD Antibody Picoband™ (A05028-1)
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No publications found for A05028-1
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