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Product Info Summary
| SKU: | PB9773 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PDK4 Antibody Picoband®
SKU/Catalog Number
PB9773
PB0823 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PDK4 Antibody Picoband® catalog # PB9773. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDK4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9773)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PDK4, different from the related mouse and rat sequences by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9773 is reactive to PDK4 in Human, Rat
Observed Molecular Weight
46 kDa
Calculated molecular weight
46.5 kDa
Background of PDK4
Pyruvate dehydrogenase lipoamide kinase isozyme 4, mitochondrial is an enzyme that in humans is encoded by the PDK4 gene. This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. In addition, PDK4 is increased in hibernation and helps to decrease metabolism and conserve glucose by decreasing its conversion to acetyl-CoA, which enters the citric acid cycle and is converted to ATP.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9773 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, human A549 whole cell, rat skeletal muscle tissue, rat heart tissue
Validation Images & Assay Conditions
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AMPK activity, protein expression and phosphorylation of PDH and PDK4 before and after fasting. Effect of seven days’ fasting on activity, phosphorylation and expression of selected proteins. White and grey bars represent means before and after seven days of fasting. White circles represent individuals before fasting and black after fasting, with dotted lines to illustrate individual change. A , B and C Activity of AMPK complex: α1β2γ1, α2β2γ1, and α2β2γ3. D Protein expression of PDH. E Phosphorylation of PDH at site 1. F Phosphorylation of PDH at site 2. G Expression of PDK4. Bars show means. Two-sided paired t tests were used for comparisons. N = 13 for (A – C , F and G ) N = 12 for ( D and E ). Abbreviation: AMPK: 5’-Adenosine monophosphate-activated protein kinase. PDH: pyruvate dehydrogenase, PDK4: pyruvate dehydrogenase kinase-4.
Index in PubMed under a CC BY license. PMID: 39747857
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Western blot analysis of PDK4 using anti-PDK4 antibody (PB9773).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat skeletal muscle tissue lysates,
Lane 5: rat heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK4 antigen affinity purified polyclonal antibody (Catalog # PB9773) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK4 at approximately 46 kDa. The expected band size for PDK4 is at 46 kDa.
Specific Publications For Anti-PDK4 Antibody Picoband® (PB9773)
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1 Customer Q&As for Anti-PDK4 Antibody Picoband®
Question
We are currently using anti-PDK4 antibody PB9773 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
The anti-PDK4 antibody (PB9773) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-06
