Product Info Summary
SKU: | M05733-1 |
---|---|
Size: | 100ug |
Reactive Species: | Human, Mouse, Rat |
Host: | Rat |
Application: | ELISA, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AHA1 Monoclonal Antibody
SKU/Catalog Number
M05733-1
Size
100ug
Form
liquid
Description
Boster Bio Anti-AHA1 Monoclonal Antibody catalog # M05733-1. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AHA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05733-1)
Host
Rat
Contents
0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
Clonality
Monoclonal
Clone Number
25F2.D9
Isotype
IgG2a Kappa
Immunogen
Recombinant Full Length Mouse Aha1 Protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~38kDa. Can run up to 45kDa on SDS Page.
Reactive Species
M05733-1 is reactive to Ahsa1 in Human, Mouse, Rat
Applications
M05733-1 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38.117kDa
Background of Ahsa1
Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of Ahsa1 using anti-Ahsa1 antibody (M05733-1).
Ahsa1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ahsa1 Antibody (M05733-1) overnight at 4°C. Biotinylated goat anti Rat IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1025) with DAB as the chromogen.
Click image to see more details
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (M05733-1) . Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (M05733-1) at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green) .
Click image to see more details
Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (M05733-1) . Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (M05733-1) at 1:1000.
Click image to see more details
Figure 4. Western blot analysis of Ahsa1 using anti-Ahsa1 antibody (M05733-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ahsa1 antigen affinity purified polyclonal antibody (Catalog # M05733-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1025) with Tanon 5200 system. A specific band was detected for Ahsa1.
Protein Target Info & Infographic
Gene/Protein Information For Ahsa1 (Source: Uniprot.org, NCBI)
Gene Name
Ahsa1
Full Name
Activator of 90 kDa heat shock protein ATPase homolog 1
Weight
38.117kDa
Superfamily
AHA1 family
Alternative Names
activator of 90 kDa heat shock protein ATPase homolog 1; AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast); C14orf3; chromosome 14 open reading frame 3; p38AHA1 Ahsa1|Ah, BC023857, p3, p38|AHA1, activator of heat shock protein ATPase 1|activator of 90 kDa heat shock protein ATPase homolog 1|AHA1, activator of heat shock 90kDa protein ATPase homolog 1|AHA1, activator of heat shock protein ATPase homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ahsa1, check out the Ahsa1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ahsa1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AHA1 Monoclonal Antibody (M05733-1)
Hello CJ!
No publications found for M05733-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AHA1 Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-AHA1 Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-AHA1 Monoclonal Antibody
Question
I was wanting to use your anti-AHA1 Monoclonal antibody for ELISA for human adrenal gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human adrenal gland identification?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
You can see on the product datasheet, M05733-1 anti-AHA1 Monoclonal antibody has been validated for ELISA, IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human adrenal gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-01
Question
I was wanting to use to test anti-AHA1 Monoclonal antibody M05733-1 on human adrenal gland for research purposes, then I may be interested in using anti-AHA1 Monoclonal antibody M05733-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Brown
Verified customer
Asked: 2019-06-03
Answer
The products we sell, including anti-AHA1 Monoclonal antibody M05733-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-03
Question
Is this M05733-1 anti-AHA1 Monoclonal antibody reactive to the isotypes of AHSA1?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
The immunogen of M05733-1 anti-AHA1 Monoclonal antibody is Mouse Aha1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-08