Anti-AHA1 Monoclonal Antibody

Product Name

Anti-AHA1 Monoclonal Antibody

View all Ahsa1 Antibodies

SKU/Catalog Number

M05733-1

Size

100ug

Form

liquid

Description

Boster Bio Anti-AHA1 Monoclonal Antibody catalog # M05733-1. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AHA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05733-1)

Host

Rat

Contents

0.02M potassium phosphate, 0.15M sodium chloride, pH7.2

Clonality

Monoclonal

Clone Number

25F2.D9

Isotype

IgG2a Kappa

Immunogen

Recombinant Full Length Mouse Aha1 Protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~38kDa. Can run up to 45kDa on SDS Page.

Reactive Species

M05733-1 is reactive to Ahsa1 in Human, Mouse, Rat

Applications

M05733-1 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

38.117kDa

Background of Ahsa1

Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Click image to see more details

Figure 1. IHC analysis of Ahsa1 using anti-Ahsa1 antibody (M05733-1).
Ahsa1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ahsa1 Antibody (M05733-1) overnight at 4°C. Biotinylated goat anti Rat IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1025) with DAB as the chromogen.

Click image to see more details

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (M05733-1) . Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (M05733-1) at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green) .

Click image to see more details

Immunoprecipitation analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (M05733-1) . Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (M05733-1) at 1:1000.

Click image to see more details

Figure 4. Western blot analysis of Ahsa1 using anti-Ahsa1 antibody (M05733-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ahsa1 antigen affinity purified polyclonal antibody (Catalog # M05733-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1025) with Tanon 5200 system. A specific band was detected for Ahsa1.

Gene/Protein Information For Ahsa1 (Source: Uniprot.org, NCBI)

Alternative Names

activator of 90 kDa heat shock protein ATPase homolog 1; AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast); C14orf3; chromosome 14 open reading frame 3; p38AHA1 Ahsa1|Ah, BC023857, p3, p38|AHA1, activator of heat shock protein ATPase 1|activator of 90 kDa heat shock protein ATPase homolog 1|AHA1, activator of heat shock 90kDa protein ATPase homolog 1|AHA1, activator of heat shock protein ATPase homolog 1

For more info on Ahsa1, check out the Ahsa1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ahsa1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Ahsa1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis