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Product Info Summary
| SKU: | M03756-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Equine, Human, Mouse, Pig, Rat |
| Host: | Chicken |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Alpha Internexin INA Intermediate Neurofilament Antibody
SKU/Catalog Number
M03756-3
Size
100ul
Description
Boster Bio Anti-Alpha Internexin INA Intermediate Neurofilament Antibody catalog # M03756-3. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Alpha Internexin INA Intermediate Neurofilament Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03756-3)
Host
Chicken
Contents
Supplied as an aliquot of IgY preparation plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Purified full length recombinant rat -internexin expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03756-3 is reactive to in Bovine, Equine, Human, Mouse, Pig, Rat
Reconstitution
Observed Molecular Weight
Calculated molecular weight
127767 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03756-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot: 1:10,000. ICC/IF and IHC: 1:1,000.
Positive Control
Validation Images & Assay Conditions
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Western blot analysis of different tissue lysates using chicken pAb to -Internexin, M03756-3, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord and [7] pig spinal cord. M03756-3 antibody reveals the -internexin protein with apparent molecular weight of 64 to 66kDa, with some variability among different species.
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Immunofluorescent analysis of a rat cerebellum section stained with chicken pAb to -internexin, M03756-3, dilution 1:5,000 in red, and costained with mouse mAb to MBP, 1:5,000 in green. Following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. The -internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons.
Specific Publications For Anti-Alpha Internexin INA Intermediate Neurofilament Antibody (M03756-3)
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